6e15

Electron Microscopy
6.2Å resolution

Handover mechanism of the growing pilus by the bacterial outer membrane usher FimD

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Chaperone protein FimC Chain: C
Molecule details ›
Chain: C
Length: 241 amino acids
Theoretical weight: 26.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P31697 (Residues: 1-241; Coverage: 100%)
Gene names: JW4279, b4316, fimC
Sequence domains:
Outer membrane usher protein FimD Chain: D
Molecule details ›
Chain: D
Length: 879 amino acids
Theoretical weight: 96.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P30130 (Residues: 1-878; Coverage: 100%)
Gene names: JW5780, b4317, fimD
Sequence domains:
Protein FimF Chain: F
Molecule details ›
Chain: F
Length: 156 amino acids
Theoretical weight: 16.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08189 (Residues: 21-176; Coverage: 100%)
Gene names: JW4281, b4318, fimF
Sequence domains: Fimbrial protein
Protein FimG Chain: G
Molecule details ›
Chain: G
Length: 158 amino acids
Theoretical weight: 16.19 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08190 (Residues: 10-167; Coverage: 100%)
Gene names: JW4282, b4319, fimG
Type 1 fimbrin D-mannose specific adhesin Chain: H
Molecule details ›
Chain: H
Length: 300 amino acids
Theoretical weight: 31.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08191 (Residues: 1-300; Coverage: 100%)
Gene names: JW4283, b4320, fimH
Sequence domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.2Å
Relevant EMDB volumes: EMD-8954
Expression system: Escherichia coli