PDBe 6cyt

X-ray diffraction
3.5Å resolution

HIV-1 TAR loop in complex with Tat:AFF4:P-TEFb

Released:

Function and Biology Details

Reactions catalysed:
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Cyclin-dependent kinase 9 Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 38.23 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 2 RZ-2014
UniProt:
  • Canonical: P50750 (Residues: 1-330; Coverage: 89%)
Gene names: CDC2L4, CDK9, TAK
Sequence domains: Protein kinase domain
Cyclin-T1 Chain: B
Molecule details ›
Chain: B
Length: 264 amino acids
Theoretical weight: 30.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 2 RZ-2014
UniProt:
  • Canonical: O60563 (Residues: 1-264; Coverage: 36%)
Gene name: CCNT1
Sequence domains: Cyclin, N-terminal domain
AF4/FMR2 family member 4 Chain: C
Molecule details ›
Chain: C
Length: 36 amino acids
Theoretical weight: 4.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHB7 (Residues: 32-67; Coverage: 3%)
Gene names: AF5Q31, AFF4, HSPC092, MCEF
Protein Tat Chain: D
Molecule details ›
Chain: D
Length: 58 amino acids
Theoretical weight: 6.78 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Spodoptera aff. frugiperda 2 RZ-2014
UniProt:
  • Canonical: P04608 (Residues: 1-57; Coverage: 66%)
Gene name: tat
RNA (5'-R(P*AP*UP*CP*UP*GP*AP*GP*CP*CP*UP*GP*GP*GP*AP*GP*CP*U)-3') Chain: N
Molecule details ›
Chain: N
Length: 20 nucleotides
Theoretical weight: 6.44 KDa

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P3221
Unit cell:
a: 148.58Å b: 148.58Å c: 104.63Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.251 0.248 0.283
Expression systems:
  • Spodoptera aff. frugiperda 2 RZ-2014
  • Escherichia coli