PDBe 6cer

X-ray diffraction
2.69Å resolution

Human pyruvate dehydrogenase complex E1 component V138M mutation

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 365 amino acids
Theoretical weight: 40.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08559 (Residues: 30-390; Coverage: 93%)
Gene names: PDHA1, PHE1A
Sequence domains: Dehydrogenase E1 component
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 331 amino acids
Theoretical weight: 36.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11177 (Residues: 31-359; Coverage: 92%)
Gene names: PDHB, PHE1B
Sequence domains:

Ligands and Environments


Cofactor: Ligand TDP 4 x TDP
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 121.76Å b: 123.919Å c: 128.279Å
α: 90° β: 118.12° γ: 90°
R-values:
R R work R free
0.194 0.192 0.234
Expression system: Escherichia coli