6c5x

X-ray diffraction
3.1Å resolution

Crystal Structure of SOCS1 in complex with ElonginB and ElonginC

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero trimer
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Elongin-B Chains: B, E
Molecule details ›
Chains: B, E
Length: 118 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15370 (Residues: 1-118; Coverage: 100%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family
Elongin-C Chains: C, F
Molecule details ›
Chains: C, F
Length: 96 amino acids
Theoretical weight: 10.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15369 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Suppressor of cytokine signaling Chains: A, D
Molecule details ›
Chains: A, D
Length: 164 amino acids
Theoretical weight: 18.97 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C0LEJ4 (Residues: 48-211; Coverage: 78%)
Gene names: SOCS1, XELAEV_18045139mg, socs1
Sequence domains:
Interleukin-6 receptor subunit beta Chain: G
Molecule details ›
Chain: G
Length: 10 amino acids
Theoretical weight: 1.2 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q00560 (Residues: 754-763; Coverage: 1%)
Gene name: Il6st

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 61.122Å b: 79.996Å c: 132.747Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.271
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided