6c31

X-ray diffraction
3Å resolution

Crystal structure of TetR family protein Rv0078 in complex with DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH tetR-type domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 214 amino acids
Theoretical weight: 23.48 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O53623 (Residues: 1-201; Coverage: 100%)
Gene name: Rv0078
Sequence domains: Bacterial regulatory proteins, tetR family
DNA (5'-D(P*AP*CP*AP*AP*GP*CP*AP*GP*AP*CP*TP*GP*CP*CP*GP*GP*TP*AP*AP*C)-3') Chains: I, K
Molecule details ›
Chains: I, K
Length: 23 nucleotides
Theoretical weight: 7.05 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided
DNA (5'-D(*GP*TP*TP*AP*CP*CP*GP*GP*CP*AP*GP*TP*CP*TP*GP*CP*TP*TP*GP*TP*AP*AP*A)-3') Chains: J, L
Molecule details ›
Chains: J, L
Length: 23 nucleotides
Theoretical weight: 7.07 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 82.039Å b: 80.04Å c: 186.66Å
α: 90° β: 90.07° γ: 90°
R-values:
R R work R free
0.206 0.202 0.247
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided