PDBe 6c1d

Electron Microscopy
3.2Å resolution

High-Resolution Cryo-EM Structures of Actin-bound Myosin States Reveal the Mechanism of Myosin Force Sensing

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Actin, alpha skeletal muscle Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 375 amino acids
Theoretical weight: 41.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Unconventional myosin-Ib Chain: P
Molecule details ›
Chain: P
Length: 729 amino acids
Theoretical weight: 84.14 KDa
Source organism: Rattus norvegicus
UniProt:
  • Canonical: Q05096 (Residues: 6-728; Coverage: 64%)
Gene names: Myo1a, Myo1b, Myr1
Sequence domains:
Calmodulin-1 Chain: R
Molecule details ›
Chain: R
Length: 148 amino acids
Theoretical weight: 16.72 KDa
Source organism: unidentified
UniProt:
  • Canonical: P0DP23 (Residues: 2-149; Coverage: 99%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Phalloidin Chain: F
Molecule details ›
Chain: F
Length: 7 amino acids
Theoretical weight: 809 Da
Source organism: Amanita phalloides

Ligands and Environments

2 bound ligands:

3 modified residues:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-7329