6c04

Electron Microscopy
3.27Å resolution

Mtb RNAP Holo/RbpA/double fork DNA -closed clamp

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 37.75 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGZ1 (Residues: 1-347; Coverage: 100%)
Gene names: MTCY13E12.10c, Rv3457c, rpoA
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1179 amino acids
Theoretical weight: 130.07 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGY9 (Residues: 7-1178; Coverage: 100%)
Gene names: MTCI376.08c, Rv0667, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1326 amino acids
Theoretical weight: 148.2 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGY7 (Residues: 1-1316; Coverage: 100%)
Gene names: MTCI376.07c, Rv0668, rpoC
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 110 amino acids
Theoretical weight: 11.78 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGY5 (Residues: 2-110; Coverage: 99%)
Gene names: MTCY21B4.07, Rv1390, rpoZ
Sequence domains: RNA polymerase Rpb6
RNA polymerase sigma factor SigA Chain: F
Molecule details ›
Chain: F
Length: 531 amino acids
Theoretical weight: 58.17 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGI1 (Residues: 1-528; Coverage: 100%)
Gene names: MTCY05A6.24, Rv2703, mysA, rpoD, rpoV, sigA
Sequence domains:
RNA polymerase-binding protein RbpA Chain: J
Molecule details ›
Chain: J
Length: 111 amino acids
Theoretical weight: 12.99 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHJ5 (Residues: 1-111; Coverage: 100%)
Gene names: Rv2050, rbpA
Sequence domains: RNA polymerase-binding protein
DNA (31-MER) Chains: H, O
Molecule details ›
Chains: H, O
Length: 31 nucleotides
Theoretical weight: 9.57 KDa
DNA (26-MER) Chains: G, P
Molecule details ›
Chains: G, P
Length: 26 nucleotides
Theoretical weight: 7.93 KDa

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.27Å
Relevant EMDB volumes: EMD-7320
Expression system: Escherichia coli