› 6bm9

X-ray diffraction
2.19Å resolution

Directed evolutionary changes in MBL super family - VIM-2 Round 10

Released:
DOI: 10.2210/pdb6bm9/pdb
PDB entry 6bm9 coloured by chain, front view.
PDB entry 6bm9 coloured by chain, side view.
PDB entry 6bm9 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Cryptic genetic variation shapes the adaptive evolutionary potential of enzymes.
Baier F, Hong N, Yang G, Pabis A, Miton CM, Barrozo A, Carr PD, Kamerlin SC, Jackson CJ, Tokuriki N
Elife 8 (2019)
PMID: 30719972

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178352 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 243 amino acids
Theoretical weight: 25.88 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: Q5U7L7 (Residues: 27-266; Coverage: 98%)
Gene name: blaVIM-2
Sequence domains: Metallo-beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2
Unit cell:
a: 128.596Å b: 41.672Å c: 156.764Å
α: 90° β: 99.41° γ: 90°
R-values:
R R work R free
0.223 0.221 0.267
Expression system: Not provided

Quick links

Citations

4 review citations

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design.
Bahr et al. (2021)
3 more

1 mention without citation

Cryptic genetic variation shapes the adaptive evolutionary potential of enzymes.
Baier et al. (2019)