PDBe 6bj8

X-ray diffraction
1.75Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, B-35 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.94 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P30685 (Residues: 25-300; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
TCR 55 alpha chain Chain: D
Molecule details ›
Chain: D
Length: 204 amino acids
Theoretical weight: 22.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
TCR 55 beta chain Chain: H
Molecule details ›
Chain: H
Length: 242 amino acids
Theoretical weight: 27.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
VAL-PRO-LEU-THR-GLU-ASP-ALA-GLU-LEU Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 986 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 194.492Å b: 61.698Å c: 92.145Å
α: 90° β: 104.87° γ: 90°
R-values:
R R work R free
0.159 0.158 0.183
Expression systems:
  • Trichoplusia ni
  • Escherichia coli
  • Not provided