PDBe 6bga

X-ray diffraction
2.31Å resolution

2B4 I-Ek TCR-MHC complex with affinity-enhancing Velcro peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
H-2 class II histocompatibility antigen, E-K alpha chain Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.62 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P04224 (Residues: 26-216; Coverage: 83%)
Sequence domains:
2B4 peptide,MHC I-Ek B chain Chain: B
Molecule details ›
Chain: B
Length: 233 amino acids
Theoretical weight: 26.59 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
T cell receptor 2B4 alpha chain Chain: C
Molecule details ›
Chain: C
Length: 220 amino acids
Theoretical weight: 24.1 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
T cell receptor 2B4 beta chain Chain: D
Molecule details ›
Chain: D
Length: 255 amino acids
Theoretical weight: 29.01 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Velcro peptide Chain: E
Molecule details ›
Chain: E
Length: 15 amino acids
Theoretical weight: 1.31 KDa
Source organism: synthetic construct
Expression system: Trichoplusia ni

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Unit cell:
a: 79.107Å b: 58.851Å c: 224.619Å
α: 90° β: 91.71° γ: 90°
R-values:
R R work R free
0.195 0.192 0.237
Expression system: Trichoplusia ni