6b0r

X-ray diffraction
1.82Å resolution

Zinc finger Domain of WT1(-KTS form) with M342R Mutation and 14+1mer Oligonucleotide with 3' Triplet TGG

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Wilms tumor protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 119 amino acids
Theoretical weight: 14.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P19544 (Residues: 321-440; Coverage: 26%)
  • Best match: P19544-4 (Residues: 321-437)
Gene name: WT1
Sequence domains: Zinc finger, C2H2 type
DNA (5'-D(*AP*GP*CP*GP*TP*GP*GP*GP*AP*GP*GP*GP*TP*TP*A)-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 15 nucleotides
Theoretical weight: 4.73 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*TP*AP*AP*CP*CP*CP*TP*CP*CP*CP*AP*CP*GP*C)-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 15 nucleotides
Theoretical weight: 4.45 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 153.94Å b: 35.113Å c: 87.148Å
α: 90° β: 110.03° γ: 90°
R-values:
R R work R free
0.209 0.208 0.234
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided