6b0q

X-ray diffraction
2.79Å resolution

Zinc finger Domain of WT1(-KTS form) with 13+1mer Oligonucleotide with 3' Triplet TGT

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Wilms tumor protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 119 amino acids
Theoretical weight: 14.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P19544 (Residues: 321-440; Coverage: 26%)
  • Best match: P19544-4 (Residues: 321-437)
Gene name: WT1
Sequence domains: Zinc finger, C2H2 type
DNA (5'-D(P*GP*CP*GP*TP*GP*GP*GP*AP*GP*TP*GP*TP*T)-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 14 nucleotides
Theoretical weight: 4.39 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*AP*AP*CP*AP*CP*TP*CP*CP*CP*AP*CP*GP*C)-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 14 nucleotides
Theoretical weight: 4.17 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P63
Unit cell:
a: 161.664Å b: 161.664Å c: 42.172Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.225 0.241
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided