PDBe 6a5q

X-ray diffraction
2Å resolution

Structure of 14-3-3 beta in complex with TFEB 14-3-3 binding motif


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein beta/alpha Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 250 amino acids
Theoretical weight: 28.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
  • Canonical: P31946 (Residues: 1-246; Coverage: 100%)
Gene name: YWHAB
Sequence domains: 14-3-3 protein
TFEB pS211-peptide Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 15 amino acids
Theoretical weight: 1.56 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3221
Unit cell:
a: 85.17Å b: 85.17Å c: 209.6Å
α: 90° β: 90° γ: 120°
R R work R free
0.187 0.186 0.23
Expression systems:
  • Escherichia coli BL21
  • Not provided