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Structures of the fully assembled Saccharomyces cerevisiae spliceosome before activation.

Bai R, Wan R, Yan C, Lei J, Shi Y
Science 360 1423-1429 (2018)
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Cited: 44 times
EuropePMC logo PMID: 29794219

Abstract

The precatalytic spliceosome (B complex) is preceded by the pre-B complex. Here we report the cryo-electron microscopy structures of the Saccharomyces cerevisiae pre-B and B complexes at average resolutions of 3.3 to 4.6 and 3.9 angstroms, respectively. In the pre-B complex, the duplex between the 5' splice site (5'SS) and U1 small nuclear RNA (snRNA) is recognized by Yhc1, Luc7, and the Sm ring. In the B complex, U1 small nuclear ribonucleoprotein is dissociated, the 5'-exon-5'SS sequences are translocated near U6 snRNA, and three B-specific proteins may orient the precursor messenger RNA. In both complexes, U6 snRNA is anchored to loop I of U5 snRNA, and the duplex between the branch point sequence and U2 snRNA is recognized by the SF3b complex. Structural analysis reveals the mechanism of assembly and activation for the yeast spliceosome.

Reviews - 5zwo mentioned but not cited (2)

  1. RNAs in the spliceosome: Insight from cryoEM structures. Zhang L, Vielle A, Espinosa S, Zhao R. Wiley Interdiscip Rev RNA 10 e1523 (2019)
  2. Structures of SF3b1 reveal a dynamic Achilles heel of spliceosome assembly: Implications for cancer-associated abnormalities and drug discovery. Maji D, Grossfield A, Kielkopf CL. Biochim Biophys Acta Gene Regul Mech 1862 194440 (2019)

Articles - 5zwo mentioned but not cited (2)

  1. A Snu114-GTP-Prp8 module forms a relay station for efficient splicing in yeast. Jia J, Ganichkin OM, Preußner M, Absmeier E, Alings C, Loll B, Heyd F, Wahl MC. Nucleic Acids Res 48 4572-4584 (2020)
  2. Sequence Divergence and Functional Specializations of the Ancient Spliceosomal SF3b: Implications in Flexibility and Adaptations of the Multi-Protein Complex. Yazhini A, Srinivasan N, Sandhya S. Front Genet 12 747344 (2021)


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  1. Roles and mechanisms of alternative splicing in cancer - implications for care. Bonnal SC, López-Oreja I, Valcárcel J. Nat Rev Clin Oncol 17 457-474 (2020)
  2. The SF3b complex: splicing and beyond. Sun C. Cell Mol Life Sci 77 3583-3595 (2020)
  3. Structural and functional modularity of the U2 snRNP in pre-mRNA splicing. van der Feltz C, Hoskins AA. Crit Rev Biochem Mol Biol 54 443-465 (2019)
  4. DEAH-Box RNA Helicases in Pre-mRNA Splicing. De Bortoli F, Espinosa S, Zhao R. Trends Biochem Sci 46 225-238 (2021)
  5. mRNA Editing, Processing and Quality Control in Caenorhabditis elegans. Arribere JA, Kuroyanagi H, Hundley HA. Genetics 215 531-568 (2020)
  6. Sequence and Evolutionary Features for the Alternatively Spliced Exons of Eukaryotic Genes. Chen SY, Li C, Jia X, Lai SJ. Int J Mol Sci 20 E3834 (2019)
  7. Cytogenetic and Genetic Abnormalities with Diagnostic Value in Myelodysplastic Syndromes (MDS): Focus on the Pre-Messenger RNA Splicing Process. Douet-Guilbert N, Soubise B, Bernard DG, Troadec MB. Diagnostics (Basel) 12 1658 (2022)
  8. The SF3b Complex is an Integral Component of the Spliceosome and Targeted by Natural Product-Based Inhibitors. Larsen NA. Subcell Biochem 96 409-432 (2021)
  9. Challenges with Approved Targeted Therapies against Recurrent Mutations in CLL: A Place for New Actionable Targets. López-Oreja I, Playa-Albinyana H, Arenas F, López-Guerra M, Colomer D. Cancers (Basel) 13 3150 (2021)

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  1. Mechanism of 5' splice site transfer for human spliceosome activation. Charenton C, Wilkinson ME, Nagai K. Science 364 362-367 (2019)
  2. A unified mechanism for intron and exon definition and back-splicing. Li X, Liu S, Zhang L, Issaian A, Hill RC, Espinosa S, Shi S, Cui Y, Kappel K, Das R, Hansen KC, Zhou ZH, Zhao R. Nature 573 375-380 (2019)
  3. Structures of the human pre-catalytic spliceosome and its precursor spliceosome. Zhan X, Zhan X, Yan C, Zhang X, Lei J, Shi Y. Cell Res 28 1129-1140 (2018)
  4. Bottom-up structural proteomics: cryoEM of protein complexes enriched from the cellular milieu. Ho CM, Li X, Lai M, Terwilliger TC, Beck JR, Wohlschlegel J, Goldberg DE, Fitzpatrick AWP, Zhou ZH. Nat Methods 17 79-85 (2020)
  5. Structural basis for conformational equilibrium of the catalytic spliceosome. Wilkinson ME, Fica SM, Galej WP, Nagai K. Mol Cell 81 1439-1452.e9 (2021)
  6. Structural insights into how Prp5 proofreads the pre-mRNA branch site. Zhang Z, Rigo N, Dybkov O, Fourmann JB, Will CL, Kumar V, Urlaub H, Stark H, Lührmann R. Nature 596 296-300 (2021)
  7. Spliceosomal Prp8 intein at the crossroads of protein and RNA splicing. Green CM, Li Z, Smith AD, Novikova O, Bacot-Davis VR, Gao F, Hu S, Banavali NK, Thiele DJ, Li H, Belfort M. PLoS Biol 17 e3000104 (2019)
  8. Dynamic protein-RNA interactions in mediating splicing catalysis. Chung CS, Tseng CK, Lai YH, Wang HF, Newman AJ, Cheng SC. Nucleic Acids Res 47 899-910 (2019)
  9. Synergistic roles for human U1 snRNA stem-loops in pre-mRNA splicing. Martelly W, Fellows B, Kang P, Vashisht A, Wohlschlegel JA, Sharma S. RNA Biol 18 2576-2593 (2021)
  10. psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation. Strittmatter LM, Capitanchik C, Newman AJ, Hallegger M, Norman CM, Fica SM, Oubridge C, Luscombe NM, Ule J, Nagai K. Nat Commun 12 1488 (2021)
  11. Herboxidiene Features That Mediate Conformation-Dependent SF3B1 Interactions to Inhibit Splicing. Gamboa Lopez A, Allu SR, Mendez P, Chandrashekar Reddy G, Maul-Newby HM, Ghosh AK, Jurica MS. ACS Chem Biol 16 520-528 (2021)
  12. Increased versatility despite reduced molecular complexity: evolution, structure and function of metazoan splicing factor PRPF39. De Bortoli F, Neumann A, Kotte A, Timmermann B, Schüler T, Wahl MC, Loll B, Heyd F. Nucleic Acids Res 47 5867-5879 (2019)
  13. Prp5-Spt8/Spt3 interaction mediates a reciprocal coupling between splicing and transcription. Shao W, Ding Z, Zheng ZZ, Shen JJ, Shen YX, Pu J, Fan YJ, Query CC, Xu YZ. Nucleic Acids Res 48 5799-5813 (2020)
  14. Activation of Prp28 ATPase by phosphorylated Npl3 at a critical step of spliceosome remodeling. Yeh FL, Chang SL, Ahmed GR, Liu HI, Tung L, Yeh CS, Lanier LS, Maeder C, Lin CM, Tsai SC, Hsiao WY, Chang WH, Chang TH. Nat Commun 12 3082 (2021)
  15. An Allosteric Network for Spliceosome Activation Revealed by High-Throughput Suppressor Analysis in Saccharomyces cerevisiae. Brow DA. Genetics 212 111-124 (2019)
  16. Blocking late stages of splicing quickly limits pre-spliceosome assembly in vivo. Mendoza-Ochoa GI, Barrass JD, Maudlin IE, Beggs JD. RNA Biol 16 1775-1784 (2019)
  17. Multi-step recognition of potential 5' splice sites by the Saccharomyces cerevisiae U1 snRNP. Hansen SR, White DS, Scalf M, Corrêa IR, Smith LM, Hoskins AA. Elife 11 e70534 (2022)
  18. Phylogenetic comparison and splice site conservation of eukaryotic U1 snRNP-specific U1-70K gene family. Fan T, Zhao YZ, Yang JF, Liu QL, Tian Y, Debatosh D, Liu YG, Zhang J, Chen C, Chen MX, Zhou SM. Sci Rep 11 12760 (2021)
  19. Rewards of divergence in sequences, 3-D structures and dynamics of yeast and human spliceosome SF3b complexes. Yazhini A, Sandhya S, Srinivasan N. Curr Res Struct Biol 3 133-145 (2021)
  20. Role of the central junction in folding topology of the protein-free human U2-U6 snRNA complex. Chu H, Perea W, Greenbaum NL. RNA 26 836-850 (2020)
  21. Adaptive transcription-splicing resynchronization upon losing an essential splicing factor. Chang SL, Wang HK, Tung L, Chang TH. Nat Ecol Evol 2 1818-1823 (2018)
  22. Identification of transient intermediates during spliceosome activation by single molecule fluorescence microscopy. Fu X, Kaur H, Rodgers ML, Montemayor EJ, Butcher SE, Hoskins AA. Proc Natl Acad Sci U S A 119 e2206815119 (2022)
  23. A UHM-ULM interface with unusual structural features contributes to U2AF2 and SF3B1 association for pre-mRNA splicing. Galardi JW, Bela VN, Jeffery N, He X, Glasser E, Loerch S, Jenkins JL, Pulvino MJ, Boutz PL, Kielkopf CL. J Biol Chem 298 102224 (2022)
  24. Domain Requirements and Genetic Interactions of the Mud1 Subunit of the Saccharomyces cerevisiae U1 snRNP. Agarwal R, Schwer B, Shuman S. G3 (Bethesda) 9 145-151 (2019)
  25. Functional analysis of Cwc24 ZF-domain in 5' splice site selection. Wu NY, Cheng SC. Nucleic Acids Res 47 10327-10339 (2019)
  26. Topology of the U12-U6atac snRNA Complex of the Minor Spliceosome and Binding by NTC-Related Protein RBM22. Ciavarella J, Perea W, Greenbaum NL. ACS Omega 5 23549-23558 (2020)
  27. Two oppositely-charged sf3b1 mutations cause defective development, impaired immune response, and aberrant selection of intronic branch sites in Drosophila. Zhang B, Ding Z, Li L, Xie LK, Fan YJ, Xu YZ. PLoS Genet 17 e1009861 (2021)
  28. Cryo-EM analyses of dimerized spliceosomes provide new insights into the functions of B complex proteins. Zhang Z, Kumar V, Dybkov O, Will CL, Urlaub H, Stark H, Lührmann R. EMBO J 43 1065-1088 (2024)
  29. Impact on splicing in Saccharomyces cerevisiae of random 50-base sequences inserted into an intron. Perchlik M, Sasse A, Mostafavi S, Fields S, Cuperus JT. RNA 30 52-67 (2023)
  30. RNA recognition by Npl3p reveals U2 snRNA-binding compatible with a chaperone role during splicing. Moursy A, Cléry A, Gerhardy S, Betz KM, Rao S, Mazur J, Campagne S, Beusch I, Duszczyk MM, Robinson MD, Panse VG, Panse VG, Allain FH. Nat Commun 14 7166 (2023)
  31. U2 snRNA structure is influenced by SF3A and SF3B proteins but not by SF3B inhibitors. Urabe VK, Stevers M, Ghosh AK, Jurica MS. PLoS One 16 e0258551 (2021)


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