› 5zvh

X-ray diffraction
2.5Å resolution

The crystal structure of NSun6 from Pyrococcus horikoshii with SFG

Released:
DOI: 10.2210/pdb5zvh/pdb
PDB entry 5zvh coloured by chain, front view.
PDB entry 5zvh coloured by chain, side view.
PDB entry 5zvh coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Archaeal NSUN6 catalyzes m5C72 modification on a wide-range of specific tRNAs.
Li J, Li H, Long T, Dong H, Wang ED, Liu RJ
Nucleic Acids Res 47 2041-2055 (2019)
PMID: 30541086

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129598 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (cytosine(72)-C(5))-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 384 amino acids
Theoretical weight: 43.8 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O57712 (Residues: 5-388; Coverage: 99%)
Gene name: PH1991
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SFG 2 x SFG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 52.471Å b: 52.941Å c: 84.185Å
α: 81.49° β: 81.38° γ: 67.45°
R-values:
R R work R free
0.185 0.182 0.23
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Eukaryotic 5-methylcytosine (m⁵C) RNA Methyltransferases: Mechanisms, Cellular Functions, and Links to Disease.
Bohnsack et al. (2019)
9 more

1 mention without citation

Archaeal NSUN6 catalyzes m5C72 modification on a wide-range of specific tRNAs.
Li et al. (2019)