› 5zt7

X-ray diffraction
2.94Å resolution

SirB from Bacillus subtilis with Co2+

Released:
DOI: 10.2210/pdb5zt7/pdb
PDB entry 5zt7 coloured by chain, front view.
PDB entry 5zt7 coloured by chain, side view.
PDB entry 5zt7 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structure of sirohydrochlorin ferrochelatase SirB: the last of the structures of the class II chelatase family.
Fujishiro T, Shimada Y, Nakamura R, Ooi M
Dalton Trans 48 6083-6090 (2019)
PMID: 30778451

Function and Biology Details

Reaction catalysed: 
Siroheme + 2 H(+) = sirohydrochlorin + Fe(2+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sirohydrochlorin ferrochelatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 273 amino acids
Theoretical weight: 30.24 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: O34632 (Residues: 1-261; Coverage: 100%)
Gene names: BSU15620, sirB, ylnE
Sequence domains: CbiX

Ligands and Environments

1 bound ligand:
Ligand CO 9 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21
Unit cell:
a: 64.96Å b: 52.09Å c: 75.47Å
α: 90° β: 112.09° γ: 90°
R-values:
R R work R free
0.247 0.246 0.276
Expression system: Escherichia coli

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Citations

1 review citation

Diversity of the reaction mechanisms of SAM-dependent enzymes.
Sun et al. (2021)
2 other articles