› 5zsx

X-ray diffraction
2.2Å resolution

Catechol 2,3-dioxygenase with 3-fluorocatechol from Diaphorobacter sp DS2

Released:
DOI: 10.2210/pdb5zsx/pdb
PDB entry 5zsx coloured by chain, front view.
PDB entry 5zsx coloured by chain, side view.
PDB entry 5zsx coloured by chain, top view.
Source organism: Diaphorobacter sp. DS2
Entry authors: Mishra K, Arya CK, Subramanian R, Ramanathan G

Function and Biology Details

Reaction catalysed: 
Catechol + O(2) = 2-hydroxymuconate-6-semialdehyde
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105187 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metapyrocatechase Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 34.92 KDa
Source organism: Diaphorobacter sp. DS2
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A4V8GZK8 (Residues: 1-314; Coverage: 100%)
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Ligands and Environments

5 bound ligands:
Ligand CA 1 x CA
Ligand FE 1 x FE
Ligand 3FA 1 x 3FA
Ligand PEG 1 x PEG
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P6222
Unit cell:
a: 102.281Å b: 102.281Å c: 113.851Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.187 0.236
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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