› 5zq1

X-ray diffraction
3.1Å resolution

Crystal structure of spRlmCD with U1939loop RNA at 3.10 angstrom

Released:
DOI: 10.2210/pdb5zq1/pdb
PDB entry 5zq1 coloured by chain, front view.
PDB entry 5zq1 coloured by chain, side view.
PDB entry 5zq1 coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Unveiling the structural features that determine the dual methyltransferase activities of Streptococcus pneumoniae RlmCD.
Jiang Y, Yu H, Li F, Cheng L, Zhu L, Shi Y, Gong Q
PLoS Pathog 14 e1007379 (2018)
PMID: 30388185

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188959 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Uncharacterized RNA methyltransferase SP_1029 Chain: A
Molecule details ›
Chain: A
Length: 452 amino acids
Theoretical weight: 51.17 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q97R12 (Residues: 1-452; Coverage: 83%)
Gene name: SP_1029
Sequence domains:
RNA (5'-R(*AP*AP*AP*(MUM)P*UP*CP*CP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.47 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SAH 1 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 47.386Å b: 96.097Å c: 114.162Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.237
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

Quick links

Citations

1 mention without citation

Unveiling the structural features that determine the dual methyltransferase activities of Streptococcus pneumoniae RlmCD.
Jiang et al. (2018)