› 5zm8

X-ray diffraction
2.7Å resolution

Crystal structure of ORP2-ORD in complex with PI(4,5)P2

Released:
DOI: 10.2210/pdb5zm8/pdb
PDB entry 5zm8 coloured by chain, front view.
PDB entry 5zm8 coloured by chain, side view.
PDB entry 5zm8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
ORP2 Delivers Cholesterol to the Plasma Membrane in Exchange for Phosphatidylinositol 4, 5-Bisphosphate (PI(4,5)P2).
Wang H, Ma Q, Qi Y, Dong J, Du X, Rae J, Wang J, Wu WF, Brown AJ, Parton RG, Wu JW, Yang H
Mol Cell 73 458-473.e7 (2019)
PMID: 30581148

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190521 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxysterol-binding protein-related protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 455 amino acids
Theoretical weight: 52.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H1P3 (Residues: 49-480; Coverage: 90%)
Gene names: KIAA0772, ORP2, OSBPL2
Sequence domains: Oxysterol-binding protein

Ligands and Environments

1 bound ligand:
Ligand IEP 2 x IEP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: I422
Unit cell:
a: 156.16Å b: 156.16Å c: 199.221Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Mechanisms and regulation of cholesterol homeostasis.
Luo et al. (2020)
11 more

2 mentions without citation

Oxysterol-binding protein-like 2 contributes to the developmental progression of preadipocytes by binding to β-catenin.
Wang et al. (2021)
1 more