› 5zlt

X-ray diffraction
2.5Å resolution

Crystal structure of UDP-GlcNAc 2-epimerase NeuC complexed with UDP

Released:
DOI: 10.2210/pdb5zlt/pdb
PDB entry 5zlt coloured by chain, front view.
PDB entry 5zlt coloured by chain, side view.
PDB entry 5zlt coloured by chain, top view.
Source organism: Acinetobacter baumannii
Primary publication:
The tetrameric structure of sialic acid-synthesizing UDP-GlcNAc 2-epimerase from Acinetobacter baumannii: A comparative study with human GNE.
Ko TP, Lai SJ, Hsieh TJ, Yang CS, Chen Y
J Biol Chem 293 10119-10127 (2018)
PMID: 29764940

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine + H(2)O = N-acetyl-D-mannosamine + UDP
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103035 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 2-epimerase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 380 amino acids
Theoretical weight: 42.43 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A154EJU5 (Residues: 1-378; Coverage: 100%)
Gene names: CAS83_20140, F2P40_13620, G3N53_15425, neuC
Sequence domains: UDP-N-acetylglucosamine 2-epimerase

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand UDP 2 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 85.587Å b: 126.144Å c: 145.998Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.257
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Genomic and Phenotypic Analyses of Acinetobacter baumannii Isolates From Three Tertiary Care Hospitals in Thailand.
Loraine et al. (2020)
1 more

1 mention without citation

The tetrameric structure of sialic acid-synthesizing UDP-GlcNAc 2-epimerase from Acinetobacter baumannii: A comparative study with human GNE.
Ko et al. (2018)