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X-ray diffraction
3.2Å resolution

Crystal structure of human Enterovirus D68 RdRp in complex with NADPH

Released:
Source organism: enterovirus D68
Entry authors: Wang ML, Li L, Chen YP, Jiang H, Zhang Y, Su D

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
NTP + H(2)O = NDP + phosphate
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RdRp Chain: A
Molecule details ›
Chain: A
Length: 457 amino acids
Theoretical weight: 51.91 KDa
Source organism: enterovirus D68
Expression system: Escherichia coli
UniProt:
  • Canonical: I6TFG7 (Residues: 1731-2187; Coverage: 21%)
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P41212
Unit cell:
a: 60.683Å b: 60.683Å c: 360.518Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.248 0.314
Expression system: Escherichia coli