› 5zg8

X-ray diffraction
2.4Å resolution

Crystal Structure of TtNRS

Released:
DOI: 10.2210/pdb5zg8/pdb
PDB 5zg8 coloured by chain and viewed from the front.
PDB 5zg8 coloured by chain and viewed from the side.
PDB 5zg8 coloured by chain and viewed from the top.
Source organism: Thermus thermophilus
Entry authors: Mutharasappan N, Jain V, Sharma A, Manickam Y, Jeyaraman J

Function and Biology Details

Reaction catalysed: 
ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Asparagine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 438 amino acids
Theoretical weight: 50.86 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54263 (Residues: 1-438; Coverage: 100%)
Gene names: TTHA0708, asnS
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P3121
Unit cell:
a: 67.294Å b: 67.294Å c: 189.117Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.155 0.209
Expression system: Escherichia coli BL21(DE3)

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