X-ray diffraction
2.11Å resolution

The structure of holo- structure of DHAD complex with [2Fe-2S] cluster


Function and Biology Details

Reaction catalysed:
2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H(2)O
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Dihydroxy-acid dehydratase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 573 amino acids
Theoretical weight: 61.01 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli K-12
  • Canonical: Q9LIR4 (Residues: 36-608; Coverage: 94%)
Gene names: At3g23940, DHAD, F14O13.13, ILVD
Sequence domains: Dehydratase family

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P42212
Unit cell:
a: 135.467Å b: 135.467Å c: 65.978Å
α: 90° β: 90° γ: 90°
R R work R free
0.175 0.173 0.215
Expression system: Escherichia coli K-12