› 5zd0

Solution NMR

Solution structure of human ubiquitin with three alanine mutations in living eukaryotic cells by in-cell NMR spectroscopy

Released:
DOI: 10.2210/pdb5zd0/pdb
PDB entry 5zd0 coloured by chain, ensemble of 20 models, front view.
PDB entry 5zd0 coloured by chain, ensemble of 20 models, side view.
PDB entry 5zd0 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
High-Resolution Protein 3D Structure Determination in Living Eukaryotic Cells.
Tanaka T, Ikeya T, Kamoshida H, Suemoto Y, Mishima M, Shirakawa M, Güntert P, Ito Y
Angew Chem Int Ed Engl 58 7284-7288 (2019)
PMID: 30938016

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143231 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.46 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 55%
Refinement method: torsion angle dynamics
Expression system: Spodoptera frugiperda

Quick links

Citations

6 review citations

Protein Structure Determination in Living Cells.
Ikeya et al. (2019)
5 more