› 5zch

X-ray diffraction
2.47Å resolution

Crystal structure of OsPP2C50 I267W:OsPYL/RCAR3 with (+)-ABA

Released:
DOI: 10.2210/pdb5zch/pdb
PDB entry 5zch coloured by chain, front view.
PDB entry 5zch coloured by chain, side view.
PDB entry 5zch coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Comprehensive survey of the VxGΦL motif of PP2Cs from Oryza sativa reveals the critical role of the fourth position in regulation of ABA responsiveness.
Han S, Lee JY, Lee Y, Kim TH, Lee S
Plant Mol Biol 101 455-469 (2019)
PMID: 31541388

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179580 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein phosphatase 2C 50 Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 35.65 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6L5H6 (Residues: 58-385; Coverage: 85%)
Gene names: ABIL3, LOC_Os05g46040, OJ1741_B01.18, OSJNBa0052K01.2, Os05g0537400, PP2C50
Sequence domains: Protein phosphatase 2C
Structure domains: PPM-type phosphatase domain
Abscisic acid receptor PYL3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 175 amino acids
Theoretical weight: 20.06 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6EN42 (Residues: 30-204; Coverage: 86%)
Gene names: LOC_Os02g15640, OSJNBa0052K15.19, Os02g0255500, P0613F08.1, PYL10, PYL3, RCAR3
Sequence domains: Polyketide cyclase / dehydrase and lipid transport
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand A8S 2 x A8S
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P1
Unit cell:
a: 71.731Å b: 76.028Å c: 78.586Å
α: 62.3° β: 73.09° γ: 65.01°
R-values:
R R work R free
0.207 0.206 0.23
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Protein-protein interfaces in molecular glue-induced ternary complexes: classification, characterization, and prediction.
Rui et al. (2023)