PDBe 5zam

Electron Microscopy
5.7Å resolution

Cryo-EM structure of human Dicer and its complexes with a pre-miRNA substrate

Released:
Source organism: Homo sapiens
Related structures: EMD-6906

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Endoribonuclease Dicer Chain: A
Molecule details ›
Chain: A
Length: 1922 amino acids
Theoretical weight: 218.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9UPY3 (Residues: 1-1922; Coverage: 100%)
Gene names: DICER, DICER1, HERNA, KIAA0928
Sequence domains:
RISC-loading complex subunit TARBP2 Chain: B
Molecule details ›
Chain: B
Length: 366 amino acids
Theoretical weight: 39.09 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15633 (Residues: 1-366; Coverage: 100%)
Gene names: TARBP2, TRBP
Sequence domains: Double-stranded RNA binding motif
RNA (73-mer) Chain: C
Molecule details ›
Chain: C
Length: 73 nucleotides
Theoretical weight: 23.38 KDa
Sequence domains: let-7 microRNA precursor

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.7Å
Relevant EMDB volumes: EMD-6906
Expression system: Homo sapiens