› 5zam

Electron Microscopy
5.7Å resolution

Cryo-EM structure of human Dicer and its complexes with a pre-miRNA substrate

Released:
DOI: 10.2210/pdb5zam/pdb
PDB 5zam coloured by chain and viewed from the front.
PDB 5zam coloured by chain and viewed from the side.
PDB 5zam coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Cryo-EM Structure of Human Dicer and Its Complexes with a Pre-miRNA Substrate.
Liu Z, Wang J, Cheng H, Ke X, Sun L, Zhang QC, Wang HW
Cell 173 1191-1203.e12 (2018)
PMID: 29706542
Related structures: EMD-6906

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Endoribonuclease Dicer Chain: A
Molecule details ›
Chain: A
Length: 1922 amino acids
Theoretical weight: 218.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9UPY3 (Residues: 1-1922; Coverage: 100%)
Gene names: DICER, DICER1, HERNA, KIAA0928
Sequence domains:
RISC-loading complex subunit TARBP2 Chain: B
Molecule details ›
Chain: B
Length: 366 amino acids
Theoretical weight: 39.09 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15633 (Residues: 1-366; Coverage: 100%)
Gene names: TARBP2, TRBP
Sequence domains: Double-stranded RNA binding motif
RNA (73-mer) Chain: C
Molecule details ›
Chain: C
Length: 73 nucleotides
Theoretical weight: 23.38 KDa
Sequence domains: let-7 microRNA precursor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.7Å
Relevant EMDB volumes: EMD-6906
Expression system: Homo sapiens

Quick links

Citations

3 review citations

Double-Stranded RNA Sensors and Modulators in Innate Immunity.
Hur S. (2019)
2 more

1 mention without citation

Cancer-associated mutations in DICER1 RNase IIIa and IIIb domains exert similar effects on miRNA biogenesis.
Vedanayagam et al. (2019)