5z9o

X-ray diffraction
2.7Å resolution

The crystal structure of Cyclopropane-fatty-acyl-phospholipid synthase from Lactobacillus acidophilus

Released:
Entry authors: Pan CL, Ma YL, Wang QH

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclopropane-fatty-acyl-phospholipid synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 399 amino acids
Theoretical weight: 46.03 KDa
Source organism: Lactobacillus acidophilus NCFM
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5FJM0 (Residues: 1-393; Coverage: 100%)
Gene name: LBA1274
Sequence domains: Mycolic acid cyclopropane synthetase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P61
Unit cell:
a: 120.982Å b: 120.982Å c: 163.487Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.175 0.213
Expression system: Escherichia coli