X-ray diffraction
3.1Å resolution

Crystal structure of Arabidopsis thaliana EBS C-terminal deletion construct in complex with an H3K4me2 peptide


Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chromatin remodeling protein EBS Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 199 amino acids
Theoretical weight: 22.65 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
  • Canonical: F4JL28 (Residues: 1-199; Coverage: 85%)
Gene names: At4g22140, EBS, F1N20.240
Sequence domains:
Histone H3.1 Chains: P, Q, R
Molecule details ›
Chains: P, Q, R
Length: 15 amino acids
Theoretical weight: 1.59 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
  • Canonical: P59226 (Residues: 2-16; Coverage: 11%)
Gene names: At1g09200, At3g27360, At5g10390, At5g10400, At5g65360, F12B17_250, F12B17_260, HTR1, HTR13, HTR2, HTR3, HTR9, K1G2.8, MNA5.9, T12M4.9

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 73.464Å b: 75.696Å c: 80.357Å
α: 90° β: 115.41° γ: 90°
R R work R free
0.215 0.213 0.252
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided