› 5z8a

X-ray diffraction
1.4Å resolution

Crystal structure of GenB1 from Micromonospora echinospora in complex with JI-20A and PLP (external aldimine)

Released:
DOI: 10.2210/pdb5z8a/pdb
PDB entry 5z8a coloured by chain, front view.
PDB entry 5z8a coloured by chain, side view.
PDB entry 5z8a coloured by chain, top view.
Source organism: Micromonospora echinospora
Primary publication:
Complete reconstitution of the diverse pathways of gentamicin B biosynthesis.
Ban YH, Song MC, Hwang JY, Shin HL, Kim HJ, Hong SK, Lee NJ, Park JW, Cha SS, Liu HW, Yoon YJ
Nat Chem Biol 15 295-303 (2019)
PMID: 30643280

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C-6' aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 437 amino acids
Theoretical weight: 47.17 KDa
Source organism: Micromonospora echinospora
Expression system: Escherichia coli
UniProt:
  • Canonical: Q70KD9 (Residues: 1-417; Coverage: 100%)
Gene names: gacK, genB1, gntW
Sequence domains: Aminotransferase class-III

Ligands and Environments

2 bound ligands:
Ligand PLP 2 x PLP
Ligand JIA 2 x JIA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 77.765Å b: 88.687Å c: 116.849Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.172 0.194
Expression system: Escherichia coli

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Citations

6 citation in other articles

The Ixodes scapularis Symbiont Rickettsia buchneri Inhibits Growth of Pathogenic Rickettsiaceae in Tick Cells: Implications for Vector Competence.
Cull et al. (2021)
5 more

1 mention without citation

Complete reconstitution of the diverse pathways of gentamicin B biosynthesis.
Ban et al. (2019)