› 5z7r

X-ray diffraction
2.2Å resolution

Crystal structure of crotonase from Clostridium acetobutylicum

Released:
DOI: 10.2210/pdb5z7r/pdb
PDB entry 5z7r coloured by chain, front view.
PDB entry 5z7r coloured by chain, side view.
PDB entry 5z7r coloured by chain, top view.
Source organism: Clostridium acetobutylicum ATCC 824
Primary publication:
Structural insights into substrate specificity of crotonase from the n-butanol producing bacterium Clostridium acetobutylicum.
Kim EJ, Kim YJ, Kim KJ
Biochem Biophys Res Commun 451 431-5 (2014)
PMID: 25110148

Function and Biology Details

Reaction catalysed: 
A short-chain (3S)-3-hydroxyacyl-CoA = a short-chain trans-2-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156461 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short-chain-enoyl-CoA hydratase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 267 amino acids
Theoretical weight: 29.05 KDa
Source organism: Clostridium acetobutylicum ATCC 824
Expression system: Escherichia coli
UniProt:
  • Canonical: P52046 (Residues: 1-261; Coverage: 100%)
Gene names: CA_C2712, crt
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P3212
Unit cell:
a: 78.673Å b: 78.673Å c: 210.703Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.189 0.258
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

The Structure of LiuC, a 3-Hydroxy-3-Methylglutaconyl CoA Dehydratase Involved in Isovaleryl-CoA Biosynthesis in Myxococcus xanthus, Reveals Insights into Specificity and Catalysis.
Bock et al. (2016)
2 more

1 mention without citation

Structural Insight into Substrate Specificity of 3-Hydroxypropionyl-Coenzyme A Dehydratase from Metallosphaera sedula.
Lee et al. (2018)