› 5z7m

X-ray diffraction
2Å resolution

SmChiA sliding-intermediate with chitohexaose

Released:
DOI: 10.2210/pdb5z7m/pdb
PDB entry 5z7m coloured by chain, front view.
PDB entry 5z7m coloured by chain, side view.
PDB entry 5z7m coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Processive chitinase is Brownian monorail operated by fast catalysis after peeling rail from crystalline chitin.
Nakamura A, Okazaki KI, Furuta T, Sakurai M, Iino R
Nat Commun 9 3814 (2018)
PMID: 30232340

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139457 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitinase A Chain: A
Molecule details ›
Chain: A
Length: 546 amino acids
Theoretical weight: 59.24 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07254 (Residues: 24-563; Coverage: 100%)
Gene name: chiA
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, NDG
Carbohydrate polymer
2 bound ligands:
Ligand GOL 15 x GOL
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AichiSR BEAMLINE BL2S1
Spacegroup: C2221
Unit cell:
a: 131.41Å b: 200.73Å c: 59.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.154 0.185
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins.
Priest et al. (2021)
2 more

2 mentions without citation

Processive chitinase is Brownian monorail operated by fast catalysis after peeling rail from crystalline chitin.
Nakamura et al. (2018)
1 more