› 5z6u

X-ray diffraction
1.95Å resolution

Crystal structure of D-xylose reductase from Scheffersomyces stipitis

Released:
DOI: 10.2210/pdb5z6u/pdb
PDB entry 5z6u coloured by chain, front view.
PDB entry 5z6u coloured by chain, side view.
PDB entry 5z6u coloured by chain, top view.
Source organism: Scheffersomyces stipitis CBS 6054
Primary publication:
Structural insight into D-xylose utilization by xylose reductase from Scheffersomyces stipitis.
Son HF, Lee SM, Kim KJ
Sci Rep 8 17442 (2018)
PMID: 30487522

Function and Biology Details

Reaction catalysed: 
Xylitol + NAD(P)(+) = D-xylose + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152102 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(P)H-dependent D-xylose reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 343 amino acids
Theoretical weight: 39.14 KDa
Source organism: Scheffersomyces stipitis CBS 6054
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31867 (Residues: 1-318; Coverage: 100%)
Gene names: PICST_89614, XYL1
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 69.248Å b: 87.151Å c: 122.623Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Characterization of d-xylose reductase, XyrB, from Aspergillus niger.
Terebieniec et al. (2021)
1 more

1 mention without citation

Structural insight into D-xylose utilization by xylose reductase from Scheffersomyces stipitis.
Son et al. (2018)