5z2m

X-ray diffraction
2.14Å resolution

Structure of Orp1L/Rab7 complex

Released:
Source organism: Mus musculus

Function and Biology Details

Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Oxysterol-binding protein-related protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 138 amino acids
Theoretical weight: 15.25 KDa
Source organism: Mus musculus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q91XL9 (Residues: 1-136; Coverage: 14%)
Gene names: Orp1, Orp1a, Orp1l, Osbpl1a
Sequence domains: Ankyrin repeats (3 copies)
Structure domains: Ankyrin repeat-containing domain
Ras-related protein Rab-7a Chains: A, C
Molecule details ›
Chains: A, C
Length: 176 amino acids
Theoretical weight: 20 KDa
Source organism: Mus musculus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P51150 (Residues: 1-176; Coverage: 85%)
Gene names: Rab7, Rab7a
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 43.222Å b: 109.626Å c: 77.811Å
α: 90° β: 99.94° γ: 90°
R-values:
R R work R free
0.183 0.182 0.209
Expression system: Escherichia coli K-12