5yxu

X-ray diffraction
2.7Å resolution

an affinity enhanced T cell receptor in complex with HLA-A0201 restricted HCV NS3 peptide KLVALGINAV

Released:
Source organisms:
Entry author: Yi L

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
NTP + H(2)O = NDP + phosphate
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
T cell receptor alpha chain Chains: A, F
Molecule details ›
Chains: A, F
Length: 191 amino acids
Theoretical weight: 21.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
T cell receptor beta chain Chains: B, G
Molecule details ›
Chains: B, G
Length: 242 amino acids
Theoretical weight: 27.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
HLA class I histocompatibility antigen, A alpha chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 276 amino acids
Theoretical weight: 31.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: D, H
Molecule details ›
Chains: D, H
Length: 100 amino acids
Theoretical weight: 11.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 20-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Serine protease/helicase NS3 Chains: I, J
Molecule details ›
Chains: I, J
Length: 10 amino acids
Theoretical weight: 998 Da
Source organism: Hepacivirus C
Expression system: Escherichia coli
UniProt:
  • Canonical: P27958 (Residues: 1406-1415; Coverage: 0%)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION ENHANCE ULTRA
Spacegroup: P21
Unit cell:
a: 60.106Å b: 223.107Å c: 71.855Å
α: 90° β: 98.3° γ: 90°
R-values:
R R work R free
0.238 0.235 0.289
Expression system: Escherichia coli