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Crystal structure of C-terminal fragment of SpaD from Lactobacillus rhamnosus GG generated by limited proteolysis

X-ray diffraction
1.48Å resolution
PDB entry 5yxg coloured by chain, front view.
PDB entry 5yxg coloured by chain, side view.
PDB entry 5yxg coloured by chain, top view.
Source organism: Lacticaseibacillus rhamnosus GG
Assembly composition:
monomeric (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1
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Assembly Name: Pilus assembly protein
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Multimeric state: monomeric
Accessible surface area: 13905.53 Å2
Buried surface area: 141.11 Å2
Dissociation area: 70.56 Å2
Dissociation energy (ΔGdiss): 11.85 kcal/mol
Dissociation entropy (TΔSdiss): -0.83 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-166347
Assembly 2 (preferred)
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Assembly Name: Pilus assembly protein
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Multimeric state: monomeric
Accessible surface area: 15682.85 Å2
Buried surface area: 398.32 Å2
Dissociation area: 58.68 Å2
Dissociation energy (ΔGdiss): 9.08 kcal/mol
Dissociation entropy (TΔSdiss): -0.83 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-219531

Macromolecules

Pilus assembly protein
Chain: A
Length: 317 amino acids
Theoretical weight: 34.94 KDa
Source organism: Lacticaseibacillus rhamnosus GG
Expression system: Escherichia coli BL21(DE3)
InterPro:
Pilus assembly protein in PDB entry 5yxg, assembly 1, front view.
Pilus assembly protein in PDB entry 5yxg, assembly 1, side view.
Pilus assembly protein in PDB entry 5yxg, assembly 1, top view.
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Pilus assembly protein
Chain: B
Length: 317 amino acids
Theoretical weight: 34.91 KDa
Source organism: Lacticaseibacillus rhamnosus GG
Expression system: Escherichia coli BL21(DE3)
InterPro:
Pilus assembly protein in PDB entry 5yxg, assembly 2, front view.
Pilus assembly protein in PDB entry 5yxg, assembly 2, side view.
Pilus assembly protein in PDB entry 5yxg, assembly 2, top view.
Molecule details ›

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