5yxg

X-ray diffraction
1.48Å resolution

Crystal structure of C-terminal fragment of SpaD from Lactobacillus rhamnosus GG generated by limited proteolysis

Released:
Entry authors: Chaurasia P, Pratap S, Palva A, von Ossowski I, Krishnan V

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pilus assembly protein Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 34.94 KDa
Source organism: Lacticaseibacillus rhamnosus GG
Expression system: Escherichia coli BL21(DE3)
Pilus assembly protein Chain: B
Molecule details ›
Chain: B
Length: 317 amino acids
Theoretical weight: 34.91 KDa
Source organism: Lacticaseibacillus rhamnosus GG
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 50.106Å b: 83.16Å c: 149.403Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.195
Expression system: Escherichia coli BL21(DE3)