› 5yxg

X-ray diffraction
1.48Å resolution

Crystal structure of C-terminal fragment of SpaD from Lactobacillus rhamnosus GG generated by limited proteolysis

Released:
DOI: 10.2210/pdb5yxg/pdb
PDB 5yxg coloured by chain and viewed from the front.
PDB 5yxg coloured by chain and viewed from the side.
PDB 5yxg coloured by chain and viewed from the top.
Source organism: Lacticaseibacillus rhamnosus GG
Entry authors: Chaurasia P, Pratap S, Palva A, von Ossowski I, Krishnan V

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pilus assembly protein Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 34.94 KDa
Source organism: Lacticaseibacillus rhamnosus GG
Expression system: Escherichia coli BL21(DE3)
Pilus assembly protein Chain: B
Molecule details ›
Chain: B
Length: 317 amino acids
Theoretical weight: 34.91 KDa
Source organism: Lacticaseibacillus rhamnosus GG
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
1 modified residue:
Ligand SME 2 x SME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 50.106Å b: 83.16Å c: 149.403Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.195
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

Crystal structure of the atypically adhesive SpaB basal pilus subunit: Mechanistic insights about its incorporation in lactobacillar SpaCBA pili.
Megta et al. (2020)
1 more