5ywn

X-ray diffraction
2.04Å resolution

SsCR_L211H-NADP+

Released:

Function and Biology Details

Reaction catalysed:
Cinnamyl alcohol + NADP(+) = cinnamaldehyde + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epimerase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 37.03 KDa
Source organism: Scheffersomyces stipitis CBS 6054
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A3LWG4 (Residues: 1-334; Coverage: 100%)
Gene names: GRP3.1, PICST_32463
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 46.782Å b: 53.093Å c: 70.768Å
α: 90° β: 95.5° γ: 90°
R-values:
R R work R free
0.168 0.166 0.223
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'