› 5yvo

X-ray diffraction
1.8Å resolution

Human Glutathione Transferase Omega1 covalently bound to ML175 inhibitor

Released:
DOI: 10.2210/pdb5yvo/pdb
PDB entry 5yvo coloured by chain, front view.
PDB entry 5yvo coloured by chain, side view.
PDB entry 5yvo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Glutathione transferase Omega 1-1 (GSTO1-1) modulates Akt and MEK1/2 signaling in human neuroblastoma cell SH-SY5Y.
Saisawang C, Wongsantichon J, Robinson RC, Ketterman AJ
Proteins 87 588-595 (2019)
PMID: 30874320

Function and Biology Details

Reactions catalysed: 
Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H(2)O
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160265 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase omega-1 Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 27.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P78417 (Residues: 1-241; Coverage: 100%)
Gene names: GSTO1, GSTTLP28
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand ACT 1 x ACT
Ligand MLX 2 x MLX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P3121
Unit cell:
a: 56.744Å b: 56.744Å c: 139.356Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.166 0.203
Expression system: Escherichia coli

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Citations

3 review citations

Glutathione S-Transferases in Cancer.
Singh et al. (2021)
2 more