X-ray diffraction
2.12Å resolution

DNA polymerase IV - DNA ternary complex 6

Primary publication:
Pyrophosphate hydrolysis is an intrinsic and critical step of the DNA synthesis reaction.
Nucleic Acids Res. 46 5875-5885 (2018)
PMID: 29850882

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase IV Chains: A, F
Molecule details ›
Chains: A, F
Length: 352 amino acids
Theoretical weight: 39.59 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: Q47155 (Residues: 2-351; Coverage: 100%)
Gene names: JW0221, b0231, dinB, dinP
Sequence domains:
Structure domains:
DTN1 Chains: B, G
Molecule details ›
Chains: B, G
Length: 18 nucleotides
Theoretical weight: 5.49 KDa
Source organism: Escherichia coli
Expression system: Not provided
DTN2C Chains: C, H
Molecule details ›
Chains: C, H
Length: 19 nucleotides
Theoretical weight: 5.8 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 86.19Å b: 57.01Å c: 110.04Å
α: 90° β: 94.59° γ: 90°
R R work R free
0.191 0.189 0.232
Expression systems:
  • Escherichia coli
  • Not provided