5ysm

X-ray diffraction
1.9Å resolution

Crystal Structure Analysis of Rif16

Released:
Primary publication:
Deciphering the late steps of rifamycin biosynthesis.
Nat Commun 9 2342 (2018)
PMID: 29904078

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chain: A
Molecule details ›
Chain: A
Length: 441 amino acids
Theoretical weight: 48.79 KDa
Source organism: Amycolatopsis mediterranei U32
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3CVZ6 (Residues: 2-420; Coverage: 100%)
Gene name: AMED_0653
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 35.105Å b: 70.335Å c: 81.019Å
α: 90° β: 94.43° γ: 90°
R-values:
R R work R free
0.186 0.183 0.234
Expression system: Escherichia coli BL21(DE3)