› 5ynw

X-ray diffraction
1.95Å resolution

Crystal structure of an aromatic prenyltransferase FAMD1 from Fischerella ambigua UTEX 1903 in complex with DMASPP and INN

Released:
DOI: 10.2210/pdb5ynw/pdb
PDB entry 5ynw coloured by chain, front view.
PDB entry 5ynw coloured by chain, side view.
PDB entry 5ynw coloured by chain, top view.
Source organism: Fischerella ambigua UTEX 1903
Primary publication:
Structural insight into a novel indole prenyltransferase in hapalindole-type alkaloid biosynthesis.
Wang J, Chen CC, Yang Y, Liu W, Ko TP, Shang N, Hu X, Xie Y, Huang JW, Zhang Y, Guo RT
Biochem Biophys Res Commun 495 1782-1788 (2018)
PMID: 29229390

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxyphenylpyruvate 3-dimethylallyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 36.99 KDa
Source organism: Fischerella ambigua UTEX 1903
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: V5TDY7 (Residues: 1-322; Coverage: 100%)
Gene name: ambP3
Sequence domains: Aromatic prenyltransferase Orf2

Ligands and Environments

4 bound ligands:
Ligand DST 2 x DST
Ligand GOL 1 x GOL
Ligand 8XL 1 x 8XL
Ligand IMD 1 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: C2221
Unit cell:
a: 93.583Å b: 211.607Å c: 82.421Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.151 0.194
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Enzymatic studies on aromatic prenyltransferases.
Mori T. (2020)
2 more