5yms

X-ray diffraction
2.1Å resolution

Structural basis of glycan specificity and identification of a novel glycan binding cavity in human P[19] rotavirus

Released:
Source organism: Human rotavirus A
Entry authors: Sun X, Dandi L, Duan Z

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Outer capsid protein VP8* Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 161 amino acids
Theoretical weight: 18.25 KDa
Source organism: Human rotavirus A
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: Q9Q2P6 (Residues: 64-223; Coverage: 21%)
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, A2G, GAL
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: C2
Unit cell:
a: 179.776Å b: 129.346Å c: 86.365Å
α: 90° β: 116.07° γ: 90°
R-values:
R R work R free
0.183 0.181 0.213
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4