Function and Biology Details
Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
nsp9 (preferred)
PDBe Complex ID:
PDB-CPX-197845 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
nsp9
Molecule details ›
Chains: A, B, C, D
Length: 115 amino acids
Theoretical weight: 12.91 KDa
Source organism: Porcine coronavirus HKU15
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Coronavirus replicase NSP9
Length: 115 amino acids
Theoretical weight: 12.91 KDa
Source organism: Porcine coronavirus HKU15
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
X2G6C4 (Residues: 3378-3486; Coverage: 2%)
Sequence domains: Coronavirus replicase NSP9
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SSRF BEAMLINE BL17U1
Spacegroup:
P21
Expression system: Escherichia coli BL21(DE3)
