› 5yij

X-ray diffraction
3.18Å resolution

Structure of a Legionella effector with substrates

Released:
DOI: 10.2210/pdb5yij/pdb
PDB 5yij coloured by chain and viewed from the front.
PDB 5yij coloured by chain and viewed from the side.
PDB 5yij coloured by chain and viewed from the top.
Source organisms:
Entry authors: Feng Y, Mu Y, Wang H

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SdeA Chain: A
Molecule details ›
Chain: A
Length: 968 amino acids
Theoretical weight: 109.79 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6RCR0 (Residues: 236-1195; Coverage: 64%)
Sequence domains:
Ubiquitin Chains: C, D, G
Molecule details ›
Chains: C, D, G
Length: 78 amino acids
Theoretical weight: 8.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family

Ligands and Environments

1 bound ligand:
Ligand NAI 1 x NAI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: C2
Unit cell:
a: 107.779Å b: 145.851Å c: 103.349Å
α: 90° β: 103.82° γ: 90°
R-values:
R R work R free
0.229 0.226 0.275
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Common Mechanism for Target Specificity of Protein- and DNA-Targeting ADP-Ribosyltransferases.
Yoshida et al. (2021)
9 more

2 mentions without citation

Bacterial pseudokinase catalyzes protein polyglutamylation to inhibit the SidE-family ubiquitin ligases.
Black et al. (2019)
1 more