› 5yhm

X-ray diffraction
1.91Å resolution

Crystal structure of dehydroquinate dehydratase with tris induced oligomerisation at 1.907 Angstrom resolution

Released:
DOI: 10.2210/pdb5yhm/pdb
PDB entry 5yhm coloured by chain, front view.
PDB entry 5yhm coloured by chain, side view.
PDB entry 5yhm coloured by chain, top view.
Source organism: Acinetobacter baumannii ATCC 17978
Entry authors: Iqbal N, Kaur P, Sharma S, Singh TP

Function and Biology Details

Reaction catalysed: 
3-dehydroquinate = 3-dehydroshikimate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107169 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
3-dehydroquinate dehydratase Chains: A, B, C, D, E, F, G, H, I, J, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, L
Length: 145 amino acids
Theoretical weight: 15.89 KDa
Source organism: Acinetobacter baumannii ATCC 17978
Expression system: Escherichia coli
UniProt:
  • Canonical: A3M692 (Residues: 3-147; Coverage: 96%)
Gene names: A1S_2009, aroQ
Sequence domains: Dehydroquinase class II
Structure domains: Dehydroquinase, class II
3-dehydroquinate dehydratase Chain: K
Molecule details ›
Chain: K
Length: 147 amino acids
Theoretical weight: 16.1 KDa
Source organism: Acinetobacter baumannii ATCC 17978
Expression system: Escherichia coli
UniProt:
  • Canonical: A3M692 (Residues: 1-147; Coverage: 97%)
Gene names: A1S_2009, aroQ
Sequence domains: Dehydroquinase class II
Structure domains: Dehydroquinase, class II

Ligands and Environments

2 bound ligands:
Ligand SO4 12 x SO4
Ligand TRS 4 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 81.904Å b: 155.606Å c: 155.912Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.248
Expression system: Escherichia coli

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