X-ray diffraction
4.2Å resolution

Crystal structure of the human prostaglandin E receptor EP4 in complex with Fab and an antagonist Br-derivative


Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Prostaglandin E2 receptor EP4 subtype Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 37.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: P35408 (Residues: 4-366; Coverage: 66%)
Gene names: PTGER2, PTGER4
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Heavy chain of Fab fragment Chain: H
Molecule details ›
Chain: H
Length: 253 amino acids
Theoretical weight: 27.57 KDa
Source organism: Mus musculus
Expression system: hybrid
Light chain of Fab fragment Chain: L
Molecule details ›
Chain: L
Length: 236 amino acids
Theoretical weight: 26.61 KDa
Source organism: Mus musculus
Expression system: hybrid

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2221
Unit cell:
a: 101.97Å b: 392.23Å c: 81.08Å
α: 90° β: 90° γ: 90°
R R work R free
0.334 0.333 0.359
Expression systems:
  • Spodoptera frugiperda
  • hybrid