5yfn

X-ray diffraction
2.5Å resolution

Human isocitrate dehydrogenase 1 bound with isocitrate

Released:
Source organism: Homo sapiens
Entry authors: Nordlund P, Chen D, Jansson A, Larsson A

Function and Biology Details

Reaction catalysed:
(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate dehydrogenase [NADP] cytoplasmic Chains: A, B
Molecule details ›
Chains: A, B
Length: 421 amino acids
Theoretical weight: 47.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75874 (Residues: 1-414; Coverage: 100%)
Gene names: IDH1, PICD
Sequence domains: Isocitrate/isopropylmalate dehydrogenase

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Unit cell:
a: 111.56Å b: 92Å c: 138.835Å
α: 90° β: 109.94° γ: 90°
R-values:
R R work R free
0.176 0.173 0.226
Expression system: Escherichia coli