5yew

X-ray diffraction
3.2Å resolution

Structural basis for GTP hydrolysis and conformational change of mitofusin 1 in mediating mitochondrial fusion

Released:
Source organism: Homo sapiens

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
homo dimer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitofusin-1 Chain: A
Molecule details ›
Chain: A
Length: 421 amino acids
Theoretical weight: 47.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IWA4 (Residues: 1-364, 374-374, 375-407; Coverage: 53%)
  • Best match: Q8IWA4-2 (Residues: 1-364)
Gene name: MFN1
Sequence domains: Dynamin family
Mitofusin-1 Chains: B, C
Molecule details ›
Chains: B, C
Length: 421 amino acids
Theoretical weight: 47.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IWA4 (Residues: 1-364, 374-374, 375-407; Coverage: 53%)
  • Best match: Q8IWA4-2 (Residues: 1-364)
Gene name: MFN1
Sequence domains: Dynamin family

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P3221
Unit cell:
a: 207.942Å b: 207.942Å c: 107.893Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.223 0.257
Expression system: Escherichia coli