5yef

X-ray diffraction
2.81Å resolution

Crystal structure of CTCF ZFs2-8-Hs5-1aE

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional repressor CTCF Chains: A, B, G, J
Molecule details ›
Chains: A, B, G, J
Length: 199 amino acids
Theoretical weight: 23.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Best match: P49711 (Residues: 292-490)
Gene name: CTCF
DNA (27-MER) Chains: C, E, H, K
Molecule details ›
Chains: C, E, H, K
Length: 27 nucleotides
Theoretical weight: 8.27 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (27-MER) Chains: D, F, I, L
Molecule details ›
Chains: D, F, I, L
Length: 28 nucleotides
Theoretical weight: 8.64 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 90.115Å b: 143.064Å c: 90.233Å
α: 90° β: 119.69° γ: 90°
R-values:
R R work R free
0.225 0.224 0.254
Expression systems:
  • Escherichia coli
  • Not provided