5ybv

X-ray diffraction
2.12Å resolution

The structure of the KANK2 ankyrin domain with the KIF21A peptide

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
KN motif and ankyrin repeat domain-containing protein 2 Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 28.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Thermus thermophilus shuttle vector pTRH1T
UniProt:
  • Canonical: Q63ZY3 (Residues: 578-832; Coverage: 30%)
Gene names: ANKRD25, KANK2, KIAA1518, MXRA3, SIP
Sequence domains: Ankyrin repeats (many copies)
KN motif and ankyrin repeat domain-containing protein 2 Chain: B
Molecule details ›
Chain: B
Length: 261 amino acids
Theoretical weight: 28.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Thermus thermophilus shuttle vector pTRH1T
UniProt:
  • Canonical: Q63ZY3 (Residues: 578-832; Coverage: 30%)
Gene names: ANKRD25, KANK2, KIAA1518, MXRA3, SIP
Sequence domains: Ankyrin repeats (many copies)
Kinesin-like protein KIF21A Chains: C, D
Molecule details ›
Chains: C, D
Length: 22 amino acids
Theoretical weight: 2.66 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q7Z4S6 (Residues: 1146-1167; Coverage: 1%)
Gene names: KIAA1708, KIF2, KIF21A

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P1
Unit cell:
a: 45.956Å b: 52.834Å c: 53.478Å
α: 73.02° β: 89.67° γ: 84.91°
R-values:
R R work R free
0.192 0.188 0.235
Expression systems:
  • Escherichia coli-Thermus thermophilus shuttle vector pTRH1T
  • Not provided